Sunday, July 29, 2007
P21

Optimization of retinal production from beta-carotene by Mus musculus beta-carotene 15,15'-monooxygenase

Yeong-Su Kim1, Min-Sung Kwak1, Hye-jung Kim1, Seon-Won Kim2, and Deok-Kun Oh1. (1) Department of Bioscience and biotechnology, Konkuk University, Seoul, South Korea, (2) Division of Applied Life Science, Gyeongsang National University, 900 Gajwadong, Jinju, 660-701, South Korea

Optimization of  retinal production from β-carotene by Mus musculus β-carotene 15,15'-monooxygenase

Yeong-Su Kim1, Min-Sung Kwak1, Hye-Jung Kim1,Seon-Won Kim2 Deok-kun Oh1

1Department of Bioscience and biotechnology, Konkuk University, Seoul 143-701, Republic of Korea

2Division of Applied Life Science (BK21), EB-NCRC Gyeongsang National University, Jinju 660-701, Republic of Korea


The β-carotene 15,15'-monooxygenase, converts β-carotene to retinal, catalyzes in the synthesis of vitamin A from β-carotene. The enzymatic production of retinal from β-carotene was investigated using recombinant β-carotene 15,15'-monooxygenase from mus musculus. The substrate solution was prepared by emulsion method using chloroform and tween 40. The optimum concentration of detergent was 5 % tween 40. The concentrations of substrate and enzyme for retinal production were optimal at 200 µ·/µ¤ of β-carotene and 0.2 units/mL of enzyme, respectively. Under the optimum conditions, The β-carotene 15,15'-monooxygenase produced approximately 75 µ·/µ¤ retinal after 12 h of reaction time with a productivity of 6.25 µ·/µ¤·h.


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