Monday, July 30, 2007
P120

Antifungal agents from tropical isolates of Aureobasidium pullulans

Sehanat Prasongsuk1, Pacharawan Deenarn1, Pongtharin Lotrakul1, Douglas E. Eveleigh2, and Hunsa Punnapayak1. (1) Plant Biomass Utilization Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University, Payathai Road, Pathumwan, Bangkok, Thailand, (2) Department of Biochemistry and Microbiology, Rutgers University, Lipman Hall, 76 Lipman Drive, New Brunswick, NJ 08901

A number of tropical black yeast Aureobasidium spp. was successfully isolated from bathroom surfaces in several areas of Bangkok, Thailand. They all were positively identified as A. pullulans according to their morphological, physiological and exopolysaccharide characteristics. All strains were tested for their capabilities to produce antifungal agents by culturing them in glucose production medium for 2 days and the growing cells were subsequently transferred into another production medium (10% w/v glucose) for another 3 days. Cells were harvested and extracted by 95% ethanol. The extracts were evaporated, dissolved in DMSO, and tested for antifungal activities against Aspergillus spp. using paper disc diffusion method and conidial germination test. Extract from A. pullulans strain BM1 was inhibitory to growth of A. flavus, A. fumigatus, and A. terreus while the extract from strain KT1 inhibited A. fumigatus and A. terreus. Only A. terreus was inhibited by extracts of strains HKW1 and HKW2. TLC analysis revealed banding of extracts showed similar Rf to that of aureobasidin A and corresponded to the antifungal activities observed.   


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