Sunday, July 29, 2007
P17

Regio- and Stereospecific Production of 10-Hydroxy-12(Z)-octadecenoic acid from Linoleic Acid by Stenotrophomonas nitritireducens

In-Sik Yu1, Soo-Jin Yeom1, Hye-Jung Kim1, Jung-Kul Lee2, Yong-Hwi Kim3, and Deok-Kun Oh1. (1) Department of Bioscience and biotechnology, Konkuk University, Seoul, South Korea, (2) Department of chemical engineering, Konkuk University, Seoul, South Korea, (3) Department of food science and technology, Sejong univ., Seoul, South Korea

An isolated strain that converted linoleic acid into 10-hydroxy-12(Z)-octadecenoic acid was identified as Stenotrophomonas nitritireducens by sequencing the full 16S rRNA, constructing a phylogenetic tree, and evaluating its nitrite reduction. S. nitritireducens had specificity with unique regio- and stereospecificity for C16 and C18 cis-9-unsaturated fatty acids only. These were converted into their 10-hydroxy fatty acids without detectable by-products. Of the cis-9-unsaturated fatty acids, the strain showed highest specificity for linoleic acid. With 20 g/l of cells, 15 g/l of 10-hydroxy-12(Z)-octadecenoic acid, a material used for natural flavor production, was produced from 20 g/l of linoleic acid at 30ºC and pH 7.5 after 2 h, yielding a productivity of 7.5 g/L·h. This is the highest reported production of a hydroxy fatty acid.


Web Page: www.konkuk.ac.kr/~deokkun

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