Sunday, July 29, 2007
P89

Enhanced b-carotene production from recombinant E. coli harboring an engineered isoprenoid precursor pathway with mevalonate addition

Sang-Hwal Yoon1, Hye-Min Park1, Sook-Hee Lee1, Eun-Gyeong Lee1, Hee-Kyoung Ryu1, Chong-Long Wang1, Hee-Jung Jang1, Ji-Seon Park1, Amitabha Das1, Deok-Kun Oh2, and Seon-Won Kim1. (1) Division of Applied Life Science, Gyeongsang National University, 900 Gajwadong, Jinju, 660-701, South Korea, (2) Department of Bioscience and Biotechnology, Konkuk University, Seoul, 143-503, South Korea

β-Carotene synthesis plasmid pT-HB was used for cotransformation with pSdxs and pSSN12Didi which increased IPP and DMAPP synthesis. β-Carotene production significantly increased 1.5-fold with the amplification of the dxs gene through pSdxs and 4-fold with the mevalonate bottom pathway of pSSN12Didi in the presence of 3.3 mM mevalonate. The pT-DHB, constructed by integrating the dxs gene into pT-HB, was used for co-transformation of E. coli harboring pSSN12Didi, resulting into β-carotene production of 141 mg/L. Recombinant E. coli harboring pT-DHB and pSSN12Didi was used to maximize β-carotene production by adjusting the available amounts of glycerol, a carbon source, and mevalonate, the precursor of the mevalonate bottom pathway. When recombinant E. coli was given 16.5 mM mevalonate and 2.5% (w/v) glycerol, β-carotene production of 503 mg/L in concentration was obtained at 144 hours.

This work was supported by the EB-NCRC (Grant No. R15-2003-012-02001-0), BioGreen 21 Program (Grant No. 2005041034590), Brain Pool program and BK21 program of Korea.


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