Thursday, August 2, 2007 - 8:30 AM
S154
Optimization of hydrolysates for insect and mammalian cell culture applications
Juliet Lobo-Alfonso, R&D Department, GIBCO, Invitrogen, 3175 Staley Road, Grand Island, NY 14072
Protein hydrolysates are used as total/partial serum replacement and also as media additives to enhance productivity of animal and insect cells in culture. With increased regulatory concerns over potential transmission of adventitious contaminants associated with serum and other animal-derived components, interest in using protein hydrolysates as serum replacements or nutrient supplements has grown substantially. This presentation reviews the chemistry and biology of various protein hydrolysates derived from animal, plant and microbial sources. Strategies for customizing and optimizing nutrients for specialized applications and general benefits and limitations of protein hydrolysates for biopharmaceutical production will also be discussed.
This presentation will further highlight our work in reducing the potential lot- to –lot variability of hydrolysates in protein-free, chemically defined and animal component-free formulations. Discussion of problems and solutions we have encountered should be instructive for those wishing to optimize cell culture systems