Monday, July 30, 2007
P2

Effects of lanthanum ion on the growth of Taxus chinensis cells and the biosynthesis of taxol

Fei Yu1, Jianping Wu2, and Roger Ruan1. (1) Department of Bioproducts and Biosystems Engineering, University of Minnesota, 1390 Eckles Ave, Saint paul, MN 55108, (2) Department of Chemical and Biochemical engineering, Zhejiang University, Zheda Road 38, Hangzhou, 310027, China

La3+, a kind of rare earth element, was used as an abiotic elicitor on stimulating taxol production by Taxus chinensis suspension cultures. The effects of various concentrations (0.5-10 mg/L) of La3+ and the elicitation time on taxol production were studied in detail. The greatest accumulation of taxol was occurred when La3+ was added to cultures at a concentration of 2.5 mg/L on day 12 of the culture cycle, but cell growth was thereby arrested. Combination of adsorbing taxol with organic phase (olic acid), the secretion of taxol improved greatly from about 10.3% extracellular to 63.7%. The taxol productivity was enhanced to 14.2 mg/L, about 5.4 fold of the control value.