Monday, July 30, 2007
P56
Optimization of Culture Medium for Rifamycin SV Production by Amycolatopsis mediterranei MM2 using Statistical Designs
Hae Joong Shin, Sung Bae Kim, and Chang-Joon Kim. Department of Chemical & Biological Engineering, Gyeongsang National University, 900 Gazwa-Dong, Jinju, 660-701, South Korea
The statistical optimization of the medium components for the production of rifamycin SV by Amycolatopsis mediterranei MM2 was investigated. In order to maximize the rifamycin SV yield, first, a Plakett-Burman experimental design (PBD) was used to screen the medium components among 11 nutrients. Glycerol and yeast extract were found to significantly influence the yield of rifamycin SV. Then, a central-composite experimental design (CCD) was used to optimize the concentrations of the screened components obtained from the PBD and to predict the mutual interactions between the screened components. The predicted optimal concentrations of glycerol and yeast extract were found to be 43.8 g/L and 9.5 g/L, respectively. At this optimum point, the predicted yield of rifamycin SV was 490.5 mg/L, whereas the corresponding experimental yield was 480.3±43.8 mg/L.
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