Sunday, July 29, 2007
P49
Enhanced production of cellulase-free thermostable xylanase by Bacillus pumilus ASH and its potential application in paper industry
Jitender Sharma, Bindu Battan, and Saurabh S. Dhiman. Biotechnology, Kurukshetra University, Kurukshetra, India
A very high level of cellulase-free, thermostable xylanase has been produced from newly isolated strain of Bacillus pumilus under submerged fermentation in a basal medium supplemented with wheat bran (2%w/v) pH 8.0 and at 37ºC. After optimization of various production parameters, an increase of nearly 13 fold in xylanase production (5407 IU/ml) was achieved. The produced xylanase is stable in neutral to alkaline pH region at 70ºC. The suitability of this xylanase for use in the bioleaching of eucalyptus Kraft pulp was investigated. A xylanase dose of 5 IU/g of oven dried pulp of 10% consistency exhibited the optimum bleach boosting of the pulp at pH 7.0 and 60ºC after 150 min of treatment. An increase of 5% in brightness along with an increase of 21% and 28% in whiteness and fluorescence respectively, whereas 18% decrease in the yellowness of the biotreated pulp was observed. Enzyme treated pulp when subjected to chemical bleaching, resulted in 20 % reduction in chlorine consumption and up to 10% reduction in consumption of chlorine dioxide. Also a reduction of about 16% in kappa number and 19% in permanganate no., along with a significant improvement in various pulp properties viz. viscosity, tensile strength, breaking length, burst factor, burstness, tear factor and tearness were observed in comparison to the conventional chemical bleaching.
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