Conversion Technologies (Deconstruction or Synthesis): Enzyme Science & Technology I - Assays and Biochemical Characterization

Thursday, May 2, 2013: 1:00 PM-4:30 PM
Grand Ballroom I, Ballroom Level
Convener:
Elena Vlasenko - Novozymes Inc., Davis, CA
Co-Convener:
Sarah E. Hobdey - National Renewable Energy Laboratory and EERE Biomass Program, Golden, CO
• New and improved assay methods and characterization techniques for biomass depolymerizing and debranching enzymes. • Progress in understanding and applying oxidative enzymes, expansins, swollenins, and other cell wall disrupting enzymes to improve plant cell wall deconstruction. • Characterizing and improving enzyme synergy in biomass hydrolysis: cellulases, hemicellulases, accessory, and oxidative enzymes.


1:00 PM
Application of electrochemical sensors in kinetic studies of cellulolytic enzymes
Nicolaj Cruys-Bagger1, Hirosuke Tatsumi2, Kim Borch3 and Peter Westh1, (1)Department of Science, Systems and Models, Roskilde University, Roskilde, Denmark, (2)International Young Researchers Empowerment Center, Shinshu University, Matsumoto, Nagano, Japan, (3)Novozymes, Bagsvaerd, Denmark
1:25 PM
Binding and movement of Trichoderma reesei Cel7A on crystalline cellulose surfaces revealed by time-resolved, super-resolution optical imaging
Jaemyeong Jung, Materials Physics and Applications, Los Alamos National Laboratory, Los Alamos, NM, Anurag Sethi, Theoretical Division, Los Alamos National Laboratory, Los Alamos, NM, Tiziano Gaiotto, Biosciences Division, Los Alamos National Laboratory, Tina Jeoh, Biological and Agricultural Engineering, University of California, Davis, Davis, CA, Gnana Gnanakaran, Theoretical Biology and Biophysics Division, Los Alamos National Laboratory, Los Alamos, NM and Peter M. Goodwin, Material Physics and Applications, Los Alamos National Laboratory, Los Alamos, NM
1:50 PM
Optimal enzyme mixtures for total hydrolysis of pretreated materials
Anne Kallioinen1, Terhi Puranen2 and Matti Siika-aho1, (1)VTT Technical Research Centre of Finland, Espoo, Finland, (2)Roal Ltd, Rajamäki, Finland
2:15 PM
Break
2:45 PM
Fungal Cellulases and Complexed Cellulosomal Enzymes Exhibit Synergistic Mechanisms In Cellulose Deconstruction
Michael G. Resch1, Bryon S. Donahoe1, John O. Baker1, Stephen R. Decker1, Edward A. Bayer2, Michael E. Himmel1 and Gregg T. Beckham3, (1)Biosciences Center, BioEnergy Science Center, National Renewable Energy Laboratory, Golden, CO, (2)Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel, (3)National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO
3:10 PM
Understanding enzyme specificity holds the key to biorefineries
Katja Salomon Johansen1, Jenny C. Mortimer2, Paul Dupree2 and Xiaofei Li2, (1)Novozymes A/S, Bagsværd, Denmark, (2)Biochemistry, University of Cambridge, Cambridge
3:35 PM
Strong Cellulase Inhibition by Non-Xylan Plant Hemicelluloses and Their Oligomers
Rajeev Kumar, Chemical and Environmental Engineering, and Center for Environmental Research and Technology, University of California, Riverside (BioEnergy Science Center (BESC), Oak Ridge, TN), Riverside, CA and Charles E. Wyman, Center for Environmental Research and Technology and Chemical and Environmental Engineering Department, University of California Riverside, BioEnergy Science Center, Riverside, CA
4:00 PM
Myceliophthora thermophila liquefies pretreated lignocellulosic biomass at high solids loading
Sylvanus E. Ekwe, Adrian Tsang and Justin Powlowski, Centre for Structural and Functional Genomics, Concordia University, Montreal, QC, Canada
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