Paper: Fermentation performance of GLBRC yeast strains on AFEX<sup>TM</sup> pretreated corn stover for ethanol production (35th Symposium on Biotechnology for Fuels and Chemicals (April 29-May 2, 2013))

Tuesday, April 30, 2013

Exhibit Hall

Mingjie Jin¹, Cory Sarks¹, Nirmal Uppugundla¹, Margie Magyar¹, Christa Gunawan¹, Trey K. Sato², Venkatesh Balan¹ and Bruce E. Dale¹, (1)Chemical Engineering and Materials Science; Great Lakes Bioenergy Research Center, Michigan State University, Lansing, MI, (2)Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI

Ammonia Fiber Expansion (AFEX^TM) pretreated lignocellulosic feedstocks are highly digestible and highly fermentable, making them promising substrates for biofuel production. The DOE Great Lakes Bioenergy Research Center (GLBRC) has been supporting research activities to develop novel genetically engineered strains which efficiently ferment both C5 and C6 carbon sugars in AFEX^TM pretreated biomass hydrolysates to ethanol. In the present study, we evaluated the fermentation performance of GLBRC engineered Saccharomyces cerevisiae strains on AFEX^TM pretreated biomass. The yeast strains were compared with regarding xylose consumption and ethanol yield during separate hydrolysis and fermentation (SHF). The best strains were selected and further compared in the process of simultaneous saccharification and co-fermentation (SSCF) at different solids loadings on AFEX^TM pretreated corn stover. The GLBRC yeast strains showed high cell viabilities during fermentation even at a solids loading as high as 9% (w/w) glucan loading (around 24% solids loading). An ethanol titer of 51 g/L was achieved at 9% (w/w) glucan loading on AFEX^TM pretreated corn stover during SSCF.

8-65: Fermentation performance of GLBRC yeast strains on AFEXTM pretreated corn stover for ethanol production

8-65: Fermentation performance of GLBRC yeast strains on AFEX^TM pretreated corn stover for ethanol production