Tuesday, April 30, 2013
Exhibit Hall
As a series publications have reported that it was difficult to use just one super strain to realize effective one-step cellulosic ethanol production, our group brought out the idea of using restructured consortium to solve this bottleneck. A consortium named H, which can use cellulose to produce ethanol and hydrogen under anaerobic fermentation, was isolated from farm soils in Shandong Province, China. Construction details of consortium H was obtained by DGGE (Denaturing Gradient Gel Electrophoresis), then strains with outstanding cellulosic ethanol conversion capability were isolated from consortium H. To increase the ethanol conversion rate, these strains were selected to add in consortium H. The additions of different strains have the positive, negative or no effect on ethanol production and hydrogen production. Among these restructured consortium, HN-3 showed the significant improvement on ethanol yield. Ethanol yield of consortium HN-3 increased to 81% from 61%, while the highest hydrogen production rate increased to around 240mL/day/L broth from 200mL/day /L broth. Great changes were detected between consortium HN-3 and consortium H during fermentation process by T-RFLP (Terminal Restriction Fragment Length Polymorphism). Based on further bioinformatics analysis, a series of efficient artificial consortium for efficient cellulosic ethanol production are expected to obtain.