Tuesday, April 30, 2013
Exhibit Hall
Pseudomonas nitroreducens MILB-8054A isolated from petroleum-contaminated soil, immobilized on calcium alginate beads and under resting cell condition, produced biosurfactants. Immobilized cells gave best yield of 5.6 g rhamnolipid l-1 using sucrose as carbon source. Time course study using resting cells showed that 10 % v/v of palm oil (carbon source) gave the best rhamnolipid yield of 5.1g l-1 at pH 8 and temperature of 30oC. Carbon utilization by resting cells was compared with that of growing cells. The best biosurfactant recovery procedure was acetone extraction.