Tuesday, April 20, 2010
LL Conference Facility (Hilton Clearwater Beach)
Liang Zhang1, Ying Liu1, Yan Liu2, Zhengbo Yue2 and Wei Liao2, (1)Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI, (2)Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI
Trichoderma reesei is a well known filamentous fungus used for cellulases and xylanase production. Enhancing its enzyme production and improving the enzyme activity are critical to a cellulosic biorefining industry. The experimental results demonstrated that different cellulosic materials (as carbon source) have significantly influence on enzyme activities and enzyme ratios of T. reesei culture. In order to further elucidate the effects of fungal enzymes from different carbon sources on hydrolysis of cellulosic materials, this study focuses on two common cellulosic feedstocks of corn stover and switchgrass. The enzyme cocktails from different fungal cultivation (different carbon sources and other culture conditions) will be applied on the cellulosic feedstocks. A completely random design will be adopted to carry out the experiments. A statistic model will then be constructed based on the experimental results to present the impacts of fungal enzymes from different cellulosic materials on the hydrolysis, and conclude the optimal fermentation and hydrolysis conditions for individual cellulosic feedstock
