Tuesday, April 20, 2010
8-19
Ethanol production from SPORL-pretreated lodgepole pine: Mass balance and process energy efficiency
Despite many efforts have been made in cellulosic ethanol research and development, few studies provided a mass balance analysis of biomass conversion and reported ethanol production in terms of gallons (liters) per ton of biomass. Recently, a good mass balance analysis of ethanol production from AFEX-treated corn stover using S. cerevisiae 424A (LNH-ST) capable of fermenting xylose was reported with an ethanol yield of 242.7 liter/ton corn stover (62.5% theoretical). However, similar work using woody biomass can be hardly found in the literature. Furthermore, energy balance analysis has not been paid much attention.
We will report ethanol yield of 276 liters (73 gallons) per ton of wood or 72% of theoretical from lodgepole pine using sulfite pretreatment (SPORL). The recovered cellulose substrate was simultaneously saccharified and fermented (SSF) at a solids content upto 20% using commercial cellulases and S. cerevisiae D5A. The liquor stream from the pretreatment was fermented by the same yeast strain after detoxification using an XAD resin column. The SPORL pretreatment was conducted at 180oC for a period of 25 min with a liquor-to-wood ratio of 3:1 (v/w) under various chemical dosages. Mechanical and thermal energy consumption for milling and pretreatment were determined. SSF was conducted in a laboratory bioreactor with a ribbon mixer with solids content upto 20% to achieve ethanol concentration in the fermentation broth of upto about 5% after 72 hours. The mixing energy consumed during SSF was recorded. These data were used to formulate a mass and energy balance.
We will report ethanol yield of 276 liters (73 gallons) per ton of wood or 72% of theoretical from lodgepole pine using sulfite pretreatment (SPORL). The recovered cellulose substrate was simultaneously saccharified and fermented (SSF) at a solids content upto 20% using commercial cellulases and S. cerevisiae D5A. The liquor stream from the pretreatment was fermented by the same yeast strain after detoxification using an XAD resin column. The SPORL pretreatment was conducted at 180oC for a period of 25 min with a liquor-to-wood ratio of 3:1 (v/w) under various chemical dosages. Mechanical and thermal energy consumption for milling and pretreatment were determined. SSF was conducted in a laboratory bioreactor with a ribbon mixer with solids content upto 20% to achieve ethanol concentration in the fermentation broth of upto about 5% after 72 hours. The mixing energy consumed during SSF was recorded. These data were used to formulate a mass and energy balance.
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See more of The 32nd Symposium on Biotechnology for Fuels and Chemicals (April 19-22, 2010)
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See more of The 32nd Symposium on Biotechnology for Fuels and Chemicals (April 19-22, 2010)