Tuesday, April 20, 2010
8-24

Cellulolytic enzymes production by submerged culture using pre-treated lignocellulosic agro-industrial residues as substrate

Rodrigo C. Guedes, Ribeiro Jab, Silva Nmp, Santos ES, and Macedo GR. Chemical Engineering, Federal University of Rio Grande do Norte, NT-Campus Universitário, Lagoa Nova, Natal, Brazil

Trichoderma reesei ATCC 2768 was grown in submerged culture for cellulase production using as substrates the following lignocelulosic residues: cashew peduncle bagasse and coconut bagasse. These substrates were tested by three different routes: (1) not treated, (2) treated with NaOH (1M) and (3) with high pressure explosion. The results showed that T.reesei ATCC 2768 presented greater cellulase production in a culture containing cashew peduncle bagasse treated with NaOH (2.16 IU / mL of CMCase and 0.21 IU / mL FPase), where the convertion of the cellulose in total reducing sugar (TRS) form was 98%, compared to the high pressure explosion treatment (0.85 IU / mL of CMCase and 0.17 IU / mL FPase), which presented 62% of TRS. The cashew peduncle bagasse without treatment showed a lower activity compared to the treated bagasse (0.53 IU / mL of CMCase and 0.15 IU / mL FPase). Cellulase production is lower in culture containing coconut residue treated with 1M NaOH (0.48 IU / mL of CMCase and 0.07 IU / mL FPase) and reached 49.9% convertion. Coconut bagasse without treatment did not present enzymatic activity. The cell concentrations to both substrates were greater when using NaOH (1 M) treatment than high pressure explosion one. It was observed that the cashew peduncle bagasse is a potential inducer to the cellulolytic enzymes synthesis, presenting better performing than coconut bagasse. It was also observed that the treatment improves enzymes synthesis process.Key-words: cashew peduncle bagasse; coconut bagasse;  Trichoderma reesei ATCC 2768; cellulases