Sunday, May 3, 2009
3-40
Biotransformation of Coffee Pulp/Husk by ligniculous fungi
Maria P. Sierra-Gomez and Patricia Ortiz-Bermúdez. Chemical Engineering, University of Puerto Rico- Mayaguez Campus, PO Box 5391, Mayaguez, PR 00681
Agro-industrial residues have been used as efficient substrates in several bioprocesses, the utilization of these residues as substrates for subsequent bioprocess contribute to solve pollution problems, and also is a way to improve the management of sources, which will be conducted to integrated processes, where the “wastes” could be used for example to generate energy, allowing a total sustainability of the process. In Puerto Rico, coffee is one of the most important crops, where commonly are processed by wet method. As consequence of this, is necessary establish new treatments to reduce the pollution from this source, because coffee Pulp/Husk contain a toxic (caffeine) that limited its use for animals. In the present study will be evaluated twelve strains of ligniculuos filamentous fungi (Gloeophyllum trabeum; Pleurotus ostreatus; Phanerochaete chrysosporium; Alternaria alternate; Chaetomium globosu; Microsphaeropsis sp.;Phialocephala dimorphospora; Lecythophora hoffmannii; Ceriporiopsis subvermispora; Xylaria polymorpha; Daldinia concentrica; Postia placenta) which will be maintained in PDA (Potato Dextrose Agar) used for the degradation of caffeine and lignin from coffee pulp/husk.
A Solid State Fermentation will be carried out. Experiments will be conducted with a pH (4.5-5.5) and moisture (60-70%) and on the addition of nutrient solution. Content of lignin, caffeine and lipids will be monitored. Caffeine and Lignin will be measure by HPLC and lipids will be measure by Soxhlet method. Also, this study will be conducted to obtain some by-products generated from fermentation. As a result the authors expect obtain a free caffeine biomass to use as animal feed and, oil as by-product.
A Solid State Fermentation will be carried out. Experiments will be conducted with a pH (4.5-5.5) and moisture (60-70%) and on the addition of nutrient solution. Content of lignin, caffeine and lipids will be monitored. Caffeine and Lignin will be measure by HPLC and lipids will be measure by Soxhlet method. Also, this study will be conducted to obtain some by-products generated from fermentation. As a result the authors expect obtain a free caffeine biomass to use as animal feed and, oil as by-product.