Sunday, May 3, 2009
3-99
Consolidated Bioprocessing (CBP) of AFEX-Pretreated Corn Stover using Thermoanaerobacterium saccharolyticum ALK2: A Study Case using AFEX-Pretreated Biomass as the Self-Sustained Nutrient Source for Fermentatio
Ming W. Lau1, Venkatesh Balan1, Bruce Dale1, Xiongjun Shao2, Lee R. Lynd3, and Todd Llyod4. (1) Biomass Conversion Research Laboratory, Department of Chemical Engineering and Materials Science, Michigan State University, 3700 Collins Rd, Lansing, MI 48910, (2) Thayer School of Engineering at Dartmouth College, 8000 Cummings Hall, Hanover, NH 03755, (3) Thayer School of Engineering, Dartmouth College, 8000 Cummings Hall, Hanover, NH 03755, (4) Mascoma Corporation, 16 Cavendish Court, Suite 2A, Lebanon, NH 03766
Nutrient characteristic of a fermentation medium is an important aspect that determines the efficacy of fermentation. Ammonia, amino acids, vitamins and trace elements are the essential categories of nutrients which are generally required by fermenting strains at varying concentrations and these requirements might differ depending on the strain selection. Fermentation using lignocellulosic materials as carbon source offers a promising route for various bio-based chemicals production. However, the nutrient profile of the lignocellulosic hydrolysate remains unclear.
Therefore, we are analyzing important nutrient components present in hydrolysate from AFEX pretreated corn stover, both quantitatively and qualitatively; identifying limiting or excess nutrients to fundamentally understand the fermentability of AFEX hydrolysate from corn stover. Furthermore, we seek to address the issue regarding the source (feedstock, pretreatment and enzymatic hydrolysis) of the nutrients in hydrolysate. This development will enable us to (i) Evaluate the need for further nutrient reformulation of the hydrolysate and (ii) Rationally supplement (or remove) certain nutrients to achieve optimal fermentations for different configurations on bio-mediated processes i.e. Separate Hydrolysis and Fermentation/Simultaneous Saccharification and Co-fermentation (SHF/SSCF) or Consolidated Bioprocessing (CBP).
More importantly, we seek to investigate the feasibility to ferment AFEX-treated feedstock using Thermoanaerobacterium saccharolyticum ALK2, a strain capable of CBP, solely based on nutrients provided through biomass and AFEX pretreatment. Through these evaluations, we intend to establish a foundation for effective lignocellulosic fermentation with little (or no) dependence on external nutrient sources.
Therefore, we are analyzing important nutrient components present in hydrolysate from AFEX pretreated corn stover, both quantitatively and qualitatively; identifying limiting or excess nutrients to fundamentally understand the fermentability of AFEX hydrolysate from corn stover. Furthermore, we seek to address the issue regarding the source (feedstock, pretreatment and enzymatic hydrolysis) of the nutrients in hydrolysate. This development will enable us to (i) Evaluate the need for further nutrient reformulation of the hydrolysate and (ii) Rationally supplement (or remove) certain nutrients to achieve optimal fermentations for different configurations on bio-mediated processes i.e. Separate Hydrolysis and Fermentation/Simultaneous Saccharification and Co-fermentation (SHF/SSCF) or Consolidated Bioprocessing (CBP).
More importantly, we seek to investigate the feasibility to ferment AFEX-treated feedstock using Thermoanaerobacterium saccharolyticum ALK2, a strain capable of CBP, solely based on nutrients provided through biomass and AFEX pretreatment. Through these evaluations, we intend to establish a foundation for effective lignocellulosic fermentation with little (or no) dependence on external nutrient sources.
Web Page: www.everythingbiomass.org