Debarati Paul1, Ranjit Kumar1, Bindu Nanduri1, Shane Burgess1, Todd French2, Ashley Brown3, Susan Bridges4, Yoginder Dandass4, William Holmes1, and Mark L. Lawrence1. (1) Basic sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, Starkville, MS 39762, (2) Dave C. Swalm School of Chemical Engineering, Mississippi State University, P.O. Box 9595, MS State, MS 39762, (3) Mississippi State Chemical Lab, Mississippi State, Mississippi state, MS 39762, (4) Institute for Digital Biology, Mississippi State University, Mississippi State, Starkville, MS 39762
Oligotropha carboxidovorans OM5 T. (DSM 1227, ATCC 49405) is a chemolithoautotrophic bacterium with the capability to utilize CO (carbon monoxide), CO2 (carbon dioxide) or syngas (gas mixture that contains varying amounts of CO and H2 generated by the gasification of organic wastes). Previous reports showed that the megaplasmid pHCG3 encoding the carbon monoxide dehydrogenase/ acetate synthase (CODH/ACS) enzyme complex is involved in the assimilation of CO or syngas. The present study aimed at sequencing and annotation of the circular chromosome to identify pathways responsible for assimilation of carbon that is fixed by chemolithoautotrophy into fatty acids. The knowledge of the complete genome also enabled comparative genomics studies with relatively close bacterial species. Interestingly, fatty acid methyl ester (FAME) analysis of O. carboxidovorans grown in the presence of acetate and syngas showed that the bacterium produces specific fatty acids that are components of biodiesel
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