Monday, April 30, 2007
3-37
Effect of metabolic engineering on downstream processing in succinic acid recovery from fermentation broth
Hyohak Song1, Yun Suk Huh2, Won Hi Hong2, and Sang Yup Lee1. (1) Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 373-1Guseong-dong, Yuseong-gu, Daejeon, South Korea, (2) Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 373-1Guseong-dong, Yuseong-gu, Daejeon, South Korea
This study presents how a strain improvement through metabolic engineering contributes to increasing purity and yield of a targeted chemical, saving running costs, and cutting down capital expenditure in downstream processing. A Mannheimia succiniciproducens LPK7 strain, in which ldhA (lactic acid), pflB (formic acid), and pta-ackA (acetic acid) genes were inactivated, shows an ability to produce a much higher amount of succinic acid and a huge decrease in the formation of by-products compared to the wild-type strain (MBEL55E). This allowed for a simple downstream process that consists only of single reactive extraction, vacuum distillation, and crystallization to yield extremely purified succinic acid (more than 99.5% purity) at a yield rate of 67.05%. On the other hand, the final purity levels and yield rates of succinic acid recovered from the fermentation broth produced by the MBEL55E strain were much lower than those of the LPK7 strain fermentation broth; even when multiple reactive extractions were performed.
[This work was supported by the Genome-based Integrated Bioprocess Project of the Ministry of Science and Technology. Further supports by the LG Chem Chair Professorship, the IBM SUR program, Microsoft, and by the KOSEF through the Center for Ultramicrochemical Process Systems are appreciated.].
[This work was supported by the Genome-based Integrated Bioprocess Project of the Ministry of Science and Technology. Further supports by the LG Chem Chair Professorship, the IBM SUR program, Microsoft, and by the KOSEF through the Center for Ultramicrochemical Process Systems are appreciated.].
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See more of The 29th Symposium on Biotechnology for Fuels and Chemicals (April 29 - May 2, 2007)
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See more of The 29th Symposium on Biotechnology for Fuels and Chemicals (April 29 - May 2, 2007)