Monday, April 30, 2007
5B-24

Enzymatic hydrolysis and ethanol production from wet-exploded wheat straw

Tania I. Georgieva1, Xiaoru Hou1, and Birgitte K. Ahring2. (1) BioCentrum-DTU, Technical University of Denmark, Building 227, DK-2800 Lyngby, Denmark, (2) Bsel, WSU, 2710 University Drive, Richland, WA 99354, Denmark

The complex physiochemical structure of lignocellulosic biomass requires an initial pre-treatment step in order to solubilised the biomass material. Recently, a new pre-treatment method, wet-explosion, has been developed at BioCentrum-DTU together with Cambi Bioethanol Aps, DK.

 The effectiveness of the pre-treatment method was evaluated by studying the enzymatic hydrolysis of pretreated wheat straw at solid concentrations up to 20% DM. Furthermore, fermentability of the pretreated wheat straw was evaluated using yeast Saccharomyces cerevisiae. Cellulose conversion obtained during Separate Hydrolysis and Fermentation and Simultaneous Saccharafication and Fermentation was compared, using batch and continuous techniques.