P79: Evaluation of extracts from apple, tomato, onion, turnip and leek to discolor an indigo carmine solution

Sunday, July 24, 2011
Grand Ballroom, 5th fl (Sheraton New Orleans)
Miguel Angel Ramos1, Myrna Solis-Oba2, Aida Solis3, Herminia I. Perez3 and Norberto Manjarrez3, (1)Universidad Tecnológica de Huejotzingo, Huejotzingo, Mexico, (2)Centro de Investigación en Biotecnología Aplicada, Instituto Politécnico Nacional, Tlaxcala, Mexico, (3)Sistemas Biológicos, Universidad Autónoma Metropolitana, México, Mexico
Actually there are environmental problems with textile effluents because dyes are non biodegradable, some processes to discolor such effluents are using physicochemical systems, in general they are expensive and produce sludge.  Peroxidases have been used to degrade different compounds such as textile dye. We tested the discoloration of indigo carmine (IC) at 100 ppm using different extracts: apple red and yellow; tomato red and green; purple onion, turnip and leek. Material were blended and centrifuged to get the extracts. Peroxidase activity was measured at different pH 3, 4, 5, 6, 7 and 8 and evaluated the ability of the extracts to discolored indigo carmine at these pHs; results are the average of three assays. The extracts which discolored the indigo carmine solution in more than 50 % were: leek leaves discolored in 54, 61 and 57 % at pH 4, 5 and 6 respectively; whereas using leek stalk extract, discoloration was 74 % at pH 3 and 73 % at pH 4. For purple onion extracts, results were 69 and 55 % using pH 3 and 5 respectively. With yellow apple extracts indigo carmine was discolored in 58, 65, 74 and 87 % at pH 5, 6, 7 and 8 respectively; whereas using red apple extracts, only at pH 7 and 8 discoloration was higher than 50 %, reporting 80 and 53 % for each pH value. This is a simple way to reuse vegetal material that is in bad state to discolor textile dyes.

 

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